[기계전공세미나] Microfluidic Cell Migration Assay To Evaluate Angiogenic Factors

1. 제 목 : Microfluidic Cell Migration Assay To Evaluate Angiogenic Factors
미세유체시스템의 세포 응용 연구

2. 연 사 : Seok Chung (Postdoctoral Associate, Dept. of Mechanical Eng. & Biological Eng. Div., MIT)

3. 일 시 : 2007년 9월 7일 (금) 16:30-17:30

4. 장 소 : 301동 302호

5. 내 용 :
본 세미나에서는 연사가 서울대학교 기계항공공학부에서 개발하였던 biomedical/biotechnological 미세시스템에 대하여 간단하게 개괄한 후, MIT에서 진행하고 있는 미세유체시스템의 세포응용연구에 대해 소개할 것이다.
MIT에서의 연구는 인체 내부의 혈관신생 현상을 연구할 목적으로 시작되었으며, 일차적으로는 암의 치료제 개발에 사용될 assay의 형태로 개발이 진행 중이다. 또한 개발된 시스템은 leukocyte 연구, 간세포와의 co-culture, 알츠하이머병 치료제 개발을 위한 신경세포의chemotaxis 연구 등으로 활용범위를 넓혀가고 있으며, biodegradable의 형태로 개발되어 tissue engineering 분야의 난제들을 풀어가는데 활용되고 있다.

Cell migration is essential to the formation of new blood vessels, cancer metastasis, would healing, and arthritis. To regulate migration or new capillary growth from the vascular system, various mechanical and biochemical factors have been assessed and proposed, but much remains to be learned. Using the technology of microfluidics, a powerful new tool was developed to evaluate cell migration and capillary growth directly
from in intact cell monolayer.

Endothelial cells were seeded and cultured in a central microfluidic channel to form a confluent cell monolayer.
The central channel has collagen scaffolds on both sides that cells can migrate into under precisely controlled conditions of mechanical and chemical angiogenic factors (e.g., fluid shear stress, interstitial flow, scaffold stiffness and fixed gradients of growth factors (VEGF, S1P)). To demonstrate the capabilities of this assay, we applied VEGF to the side channel adjacent to one scaffold, generating a gradient in VEGF on one side of the central cell-seeded channel. The other side channel was filled with cell culture media as a control. During several days of culture, the length and area of migrating cells into the scaffolds were observed and qauntified. Cells in contact with the VEGF gradient were highly active and rapidly migrated into the scaffold, but cells in contact with the control scaffold were more restrained and demonstrated markedly less migration after two days.

The system is suitable for quantification and comparison studies, and avoids many of the drawbacks of other methods using the scratch assay or defined shaped patterns. Since the three dimensional migration occurs perpendicular to the cell monolayer the results more closely mimic the in-vivo situation compared to other, two-dimensional and single cell assays.

The new assay has numerous applications in the study of angiogenesis or cell migration into a three-dimensional scaffold under conditions of a precisely controlled mechanical and biochemical environment. It can be also suitable to quantify the motion of leukocytes or cancerous cells transmigrating across an endothelial cell monolayer.

6. 연사약력 :
2005~현재 Postdoctoral Associate, Dept. of Mechanical Eng. & Biological Eng. Div., MIT
2002~2005 Team manager, Digital Bio Technology Co. (현 나노엔텍)
2002 서울대학교 기계항공공학부 박사
1996~2000 서울대학교 마이크로시스템기술센터 조교
1998 서울대학교 기계설계학과 석사
1996 서울대학교 기계설계학과 학사

7. 문 의 : 기계항공공학부 김호영 교수(☏ 880-9286)