[기계전공대학원세미나] (4월 24일) Mechanotaxis modulation of endothelial cells

1. 제 목 : Mechanotaxis modulation of endothelial cells

2. 일 시 : 2009년 4월 24일 (금) 16:30-17:30

3. 연 사 : 신현정 교수(Jennifer H. Shin, KAIST 기계항공시스템학부)

4. 장 소 : 301동 105호 강의실

5. 연사약력 :
2005 - 현재 KAIST 기계항공시스템학부 조교수
2004 Ph.D. MIT 기계공학과
2000 M.S. MIT 기계공학과
1998 B.S. MIT 기계공학과

6. 내용요약 : Vascular endothelial cell migration, which plays an important role in vascular remodeling, is known to be regulated by hemodynamic forces in the blood vessels. When shear stress is applied on mouse microvessel endothelial cells (B.end3) in vitro, cells exhibit anti-parallel migration behavior to the direction of the flow. To determine how shear stress magnitude influences mechanotaxis of the cells, endothelial cells were exposed to different magnitudes of uni-directional shear stress at 2, 6, and 15 dynes/cm2. While higher flow rate reduces the speed of the motility, horizontal component of the velocity parallel to the flow increases with the flow rate, indicating the higher alignment of cells in the direction parallel to the flow at a higher shear stress. In addition, cells seeded on softer substrates, whose elastic modulus is comparable to that of the blood vessels, show enhanced directional persistence when compared to those seeded on stiffer substrates. The higher directionality accompanies with increased stress fiber formation and focal adhesion turn over, exhibiting higher mechanotaxis behavior. Therefore, the increased stiffness in the vessel may interfere with the mechano-sensing mechanism of the endothelial cells, resulting in reduced mechanotaxis in response to hemodynamic shear stress. This ECM stiffness dependent migration behavior can further elucidate the endothelial cell remodeling and wound healing in pathologically hardened vessels as well as endothelialization of stents and grafted tissues.

7. 문 의 : 기계항공공학부 전누리 교수(☏ 880-7111)