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[기계] Super-resolution wide-field total internal reflection microscopy by use of standing evanescent
1. 제목 : Super-resolution wide-field total internal reflection microscopy by use of standing evanescent waves
2. 일시 : 2008년 12월 5일 (금) 15:00
3. 장소 : 서울대학교 301동 15층 세미나실 (1512호)
4. 연사 : 정의헌 박사 (Harvard Medical School)
5. 약력 :
2006 Ph.D. MIT
현 재 Post-doc., Department of Radiation Oncology,
Massachusetts General Hospital and Harvard Medical School
6. 내용요약 : Optical microscopy has been an indispensable tool in biomedical research allowing the study of dynamical processes in biological systems. However, the resolution is fundamentally limited by the wave nature of light and has been remained a major challenge demanding further improvements in resolution. In this talk, I will highlight the development of super-resolution microscopy based on total internal reflection geometry. The shallow penetration depth of evanescence field ( < 100 nm) ensures a thin imaging region resulting in low background fluorescence. The standing-wave total internal reflection fluorescence (SW-TIRF) microscopy is developed using evanescent standing wave illumination with sinusoidal, high-spatial frequency fringe patterns onto the specimen. On the other hand, the field enhancement effect of the surface plasmon resonance in the presence of thin metal film on the substrate can add additional strong fluorescence excitation. The combination of surface plasmon resonance fluorescence imaging and SW-TIRF microscopy resulted in alternative high-resolution microscopy modality. The standing-wave surface plasmon resonance fluorescence (SW-SPRF) microscopy is a novel wide-field super-resolution imaging technique by use of evanescent standing waves of surface plasmon. As in any high resolution interference microscopy, minute thermal and mechanical vibration can significantly affect microscope precision and accuracy. To minimize the undesirable drift of the phase of the interference fringe, feedback control mechanism is devised and used for stabilization and control of fringe phase. The first experimental realization of super-resolution wide-field imaging will be demonstrated by imaging fluorescent microspheres, semiconductor quantum dots and
biological specimens.
7. 문의 : 기계항공공학부 조맹효 교수(☏ 880-1693)
2. 일시 : 2008년 12월 5일 (금) 15:00
3. 장소 : 서울대학교 301동 15층 세미나실 (1512호)
4. 연사 : 정의헌 박사 (Harvard Medical School)
5. 약력 :
2006 Ph.D. MIT
현 재 Post-doc., Department of Radiation Oncology,
Massachusetts General Hospital and Harvard Medical School
6. 내용요약 : Optical microscopy has been an indispensable tool in biomedical research allowing the study of dynamical processes in biological systems. However, the resolution is fundamentally limited by the wave nature of light and has been remained a major challenge demanding further improvements in resolution. In this talk, I will highlight the development of super-resolution microscopy based on total internal reflection geometry. The shallow penetration depth of evanescence field ( < 100 nm) ensures a thin imaging region resulting in low background fluorescence. The standing-wave total internal reflection fluorescence (SW-TIRF) microscopy is developed using evanescent standing wave illumination with sinusoidal, high-spatial frequency fringe patterns onto the specimen. On the other hand, the field enhancement effect of the surface plasmon resonance in the presence of thin metal film on the substrate can add additional strong fluorescence excitation. The combination of surface plasmon resonance fluorescence imaging and SW-TIRF microscopy resulted in alternative high-resolution microscopy modality. The standing-wave surface plasmon resonance fluorescence (SW-SPRF) microscopy is a novel wide-field super-resolution imaging technique by use of evanescent standing waves of surface plasmon. As in any high resolution interference microscopy, minute thermal and mechanical vibration can significantly affect microscope precision and accuracy. To minimize the undesirable drift of the phase of the interference fringe, feedback control mechanism is devised and used for stabilization and control of fringe phase. The first experimental realization of super-resolution wide-field imaging will be demonstrated by imaging fluorescent microspheres, semiconductor quantum dots and
biological specimens.
7. 문의 : 기계항공공학부 조맹효 교수(☏ 880-1693)